![]() Now the dark parts of the film are light, and the light parts are dark. Under Image>Adjustments, select Invert (or press Ctrl+I). We don't care about color information, only grayscale information, so we can discard the color information.ģ. Under Image>Mode, check the grayscale option if it's not already selected. You can scan the film as a grayscale image, and set the resolution to a medium value (300-400dpi).Ģ. This can be a cheap consumer unit, or a more expensive transparency scanner if you have access to such a beast. To start with, you'll need to scan in your xray film on a flat-bed scanner. We'll start with Adobe Photoshop as an example (you can find many references in the literature that include phrases such as "densitometry was carried out in Photoshop" in the methods section). All you really need in a program is a freehand selection tool and a way to measure the mean gray value inside the selection. The following document outlines some of those methods.įor a long time, the de-facto standard for analysis in labs that didn't want to spring for multi-thousand $$ commercial densitometry software was Adobe Photoshop or one of the competing photo editing programs. Quantifying western blots without expensive commercial quantification software.Ĭomparing the intensity of bands on a Western blot can be done in a number of ways using software that is commonly found on lab computers or freely available for download.
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